Article: Formation of strong airway irritants in mixtures of isoprene/ozone and isoprene/ozone/nitrogen dioxide. (Articles).

We evaluated the airway irritation of isoprene, isoprene/ozone, and isoprene/ozone/nitrogen dioxide mixtures using a mouse bioassay, from which we calculated sensory irritation, bronchial constriction, and pulmonary irritation. We observed significant sensory irritation (approximately 50% reduction of mean respiratory rate) by dynamically exposing the mice, over 30 min, to mixtures of isoprene and [O.sub.3] or isoprene, [O.sub.3], and N[O.sub.2]. The starting concentrations were approximately 4 ppm [O.sub.3] and 500 ppm isoprene (+ approximately 4 ppm N[O.sub.2]). The reaction mixtures after approximately 30 sec contained

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