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Article: Use of reporter genes and vertebrate DNA motifs in transgenic zebrafish as sentinels for assessing aquatic pollution. (Correspondence).
- Article from:
- Environmental Health Perspectives
- Article date:
- January 1, 2002
- Author:
CopyrightCOPYRIGHT 2002 National Institute of Environmental Health Sciences. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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In a recent paper in EHP, Mattingly and co-workers (1) showed that dioxin-treated transgenic zebrafish [having 1,905 bp of 5' flanking region of the human CYP1A1 gene, driving the jelly fish green fluorescent protein (GFP) reporter gene] displayed inducible GFP in the eye, nose, and vertebra of embryos 48 and 72 hr after fertilization. They first confirmed in zebrafish liver cells that zebrafish transcription factor proteins [e.g. the aromatic hydrocarbon receptor (AHR) and its heterodimeric binding partner the AHR nuclear transporter (ARNT)] must be capable of recognizing and binding to human aromatic hydrocarbon response elements (AHREs) present in the CYP1A1 5' ...
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