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Article: Culture method for in vitro fertilization to hatching of the squid, Loligo pealeii.
- Article from:
- The Biological Bulletin
- Article date:
- October 1, 2002
- Author:
CopyrightCOPYRIGHT 2002 Marine Biological Laboratory. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Much of what we know about cephalopod development is drawn from observing embryos in, or dissected from, naturally laid jelly strings or egg capsules (1, 2). Although embryos extracted from jelly strings survive in culture for short periods (3), reliable in vitro fertilization and culture would greatly enhance our ability to study the molecular and cellular events of fertilization and development, including pronuclear migration (4), cleavage (5), cytoplasmic movements (6), differentiation, and organogenesis. When in vitro fertilized embryos are cultured in glassware or plastic petri dishes, their development is typically arrested at the early blastoderm stage (
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