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Osmotic induction of stress-responsive gene expression in the lobster Homarus americanus.
- Article from:
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The Biological Bulletin
- Article date:
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December 1, 2002
- Author:
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Copyright informationCOPYRIGHT 2002 Marine Biological Laboratory. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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The American lobster, Homarus americanus, encounters osmotic stress throughout its life cycle. To understand the molecular basis of osmotic stress responses in vivo, we used homologous cDNA probes to characterize the mRNA patterns of lobster HSP7O (=70-kDa heat-shock protein), HSP90 (90-kDa heat-shock protein), and polyubiquitin during hypo- and hyper-osmotic stress in abdominal muscle and hepatopancreas (a digestive tissue) at 30, 60, and 120 mm of osmotic stress. Hypo- and hyper-osmotic stress significantly increased the levels of the mRNAs encoding HSP7O and HSP90 in abdominal muscle. Hyperosmotic stress increased HSP9O mRNA levels in hepatopancreas, but hypo-osmotic stress ...
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