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Article: Multienzyme multiplex PCR effective for Klebsiella pneumoniae typing.
- Article from:
- TB & Outbreaks Week
- Article date:
- April 1, 2003
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2003 APR 1 - (NewsRx.com & NewsRx.net) -- A novel multienzyme multiplex polymerase chain reaction (PCR) assay provides an effective method for typing Klebsiella pneumoniae isolates.
Researchers in the Netherland "developed and optimized a new modified amplified fragment length polymorphism (AFLP) typing method to obtain a multibanding fingerprint that can be separated by agarose gel electrophoresis."
"Both to maximize the discriminatory power and to facilitate the computer-assisted analysis, bacterial DNA was digested with four different restriction enzymes," explained A. van der Zee and coauthors at St. Elizabeth Hospital. "After ligation of adaptors ...