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Article: Blotting
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- Genetics
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CopyrightCOPYRIGHT 2003 The Gale Group Inc. This material is published under license from the publisher through the Gale Group, Farmington Hills, Michigan. All inquiries regarding rights should be directed to the Gale Group. (Hide copyright information)
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Blotting
Blotting is a common laboratory procedure in which biological molecules in a gel matrix are transferred onto nitrocellulose paper for further scientific analysis. The biological molecules transferred in this process are DNA fragments, RNA fragments, or proteins. Because the isolation and characterization of these types of materials is at the center of much molecular biology research, blotting is one of the most useful techniques in the molecular biology laboratory.
The blotting procedure is named differently depending on the type of the molecules being transferred. When the molecules to be transferred are DNA fragments, the procedure is called a
Southern blot
, named for the ...