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Article: Trypsin-like activity of membrane-bound midgut proteases from Anticarsia gemmatalis (Lepidoptera: Noctuidae)
- Article from:
- European Journal of Entomology
- Article date:
- January 1, 2005
- Author:
CopyrightCopyright Institute of Entomology 2005. Provided by ProQuest LLC. (Hide copyright information)
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Keywords. Trypsin, proteases, velvetbean caterpillar, Lepidoptera, Noctuidae, Anticarsia
Abstract. Membrane-bound proteases from preparations of the midgut of 5th instar velvetbean caterpillars, Anticarsia gemmatalis (Hübner) were obtained by resuspension of the pellet obtained after 100,000 g centrifugation. As expected of trypsin-like proteases, they hydrolyzed casein and the synthetic substrates N-α-benzoyl-L-Arg-p-nitroanilidine (L-BApNA) and N-α-p-tosyl-L-Arg methyl ester (L-TAME). Higher activities were observed at 50°C, and at pH 8.5 and 8.0 for both synthetic substrates L-BApNA and L-TAME. The membrane-bound proteases were inhibited by EDTA, phenylmethan sulphonyl ...