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Article: Analysis of coenzyme Q^sub 10^ in breast milk by high-performance liquid chromatography with coulometric detection

Article from:
Clinical Chemistry
Article date:
June 1, 2006
Author:
Copyright

E-64

P. H. Tang, M. V. Miles, P. Steele, B. S. Davidson, S. Geraghty, A. Morrow. Cincinnati Children's Hospital Medical enter, Cincinnati, OH,

Background: A recent study suggests a protective effect of breast milk against oxidative stress during the first days of life. The finding indicates that an increase in plasma coenzyme Q^sub 10^ (CoQ^sub 10^) levels in breast-led infants could be related to the exogenous supply from breast milk. Therefore, we developed a reliable method for measuring CoQ^sub 10^ levels in breast milk. Method: Breast milk sample was mixed with coenzyme Q^sub 9^ (CoQ^sub 9^, ubiquinone-9) internal standard and extracted with 1-propanol and hexane, followed by ...

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