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Article: MAT (Multi-Auto-Transformation) vector system. The onocogenes of Agrobacterium as positive markers for regeneration and selection of marker-free transgenic plants

Article from:
In Vitro Cellular & Developmental Biology
Article date:
March 1, 2001
Author:
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SUMMARY

We have developed a new transformation method called MATVS (Multi-Auto-Transformation Vector System). The oncogenes (ipt or rol genes) of Agrobacterium are used as selectable markers to regenerate transgenic cells and to select marker-free transgenic plants in the MATVS. The chimeric ipt gene or the rol genes are combined with the site-specific recombination R/RS system to remove the oncogenes from the transgenic cells after transformation. We report here the application of MATVS to transformation of tobacco, aspen, rice and snapdragon. (1) The GST-MAT vector pMAT8 has the native ipt gene and the R gene with a chemical inducible promoter (GST-II-27). Use of the GST-MAT vector ...

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