Article: Measurement of FRET Efficiency and Ratio of Donor to Acceptor Concentration in Living Cells

Article: Photobleaching-Corrected FRET Efficiency Imaging of ...
Biophysical Journal;  June 1, 2004 ;     700+ words ... ... which allows absolute measurements of FRET efficiency, E, but is not suitable for living cells), and the sensitized-emission FRET indices ... FRET, we visualize FRET in terms of true FRET efficiency images (E), which correlate linearly ...

Article: Fluorescence resonance energy transfer-based stoichiometry in ...
Biophysical Journal;  December 1, 2002 ;     700+ words ... ... interactions inside living cells. Present microscopic ... discriminate FRET efficiency and the fractions ... images to measure FRET efficiency, the relative ... in complex in living cells. FRET stoichiometry ... characteristic FRET efficiency, which, if ...

Article: Adenovirus Type-5 Entry and Disassembly Followed in Living ...
Biophysical Journal;  August 1, 2004 ;     700+ words ... ... Ad5 were weakly labeled on their capsid proteins with FRET donor and acceptor fluorophores. A progressive decrease in FRET efficiency recorded during Ad5 uptake revealed that the time course of Ad5 capsid disassembly has two sequential protein dissociation ...

Article: Lifetime of Fluorescent Pyrene Butyric Acid Probe in Single ...
Photochemistry and Photobiology;  September 1, 2004 ;     700+ words ... ... to assess oxygen concentrations in living cells. The behavior of the probe is first ... with previous observations. In single living cells, the PBA lifetime decreases with oxygen ... The probe lifetime differences between living cells and liposome suspension, especially ...

Article: Homo-FRET microscopy in living cells to measure ...
Biophysical Journal;  June 1, 2001 ;     700+ words ... ... was used to determine, in individual living cells, the spatial monomer-dimer distribution ... information on proteins in individual living cells, even when expression levels are very ... information on protein structure in living cells is a prerequisite for a full understanding ...

Article: mRNA Transcripts Can Be Detected In Living Cells.
Gene Therapy Weekly;  August 23, 2001 ;     700+ words ... ... allows detection of mRNA transcripts in living cells was reported by scientists in Japan ... resolve method to detect c-fos in living cells using a donor oligodeoxynucleotide labeled ... method for observing hybridization in living cells using fluorescence resonance energy ...

Article: Architectural dynamics in living cells program.
The Biological Bulletin;  August 1, 2003 ;     700+ words ...The Architectural Dynamics in Living Cells Program, established at the MBL by ... in biophysical inquiries directly in living cells that Dr. Inoue started at Princeton ... focuses on architectural dynamics in living cells--the timely and coordinated assembly ...