Articles > Journals > Medical journals > British Journal of Biomedical Science articles > April 2006

Article: Modified double-disc test for detection of extended-spectrum [beta]-lactamases in Escherichia coli and Klebsiella pneumoniae

Article from:
British Journal of Biomedical Science
Article date:
April 1, 2006
Author:
Copyright

ABSTRACT

This study evaluates the performance of a modified double-disc test (MDDT) for the detection of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Escherichia coli and Klebsiella pneumoniae. Ninety-six isolates of E. coli and 40 K. pneumoniae are studied for ESBL production by the National Committee for Clinical Laboratory Standards (NCCLS) combination disc tests and MDDT. A total of 112 (82%) isolates (80 [83%] E. coli, 32 [80%] K. pneumoniae) were positive for ESBL by MDDT compared to 102 (75%; 72 [75%] E. coli and 30 [75%] K. pneumoniae) by the NCCLS method. In 10 (7.4%) isolates, ESBLs were detected only by MDDT. Twenty-four (17.6%) isolates were negative for ...

Read all of this article with a FREE trial

Related newspaper, magazine, and journal articles:

Article: Evaluation of CIVA agar for rapid detection of extended spectrum ...
Indian Journal of Medical Research;  February 1, 2008 ;     700+ words ... ... contributed to uncontrolled spread of ESBL producers and related treatment ... Standard methods for detection of ESBL in clinical isolates namely ... test, National Committee for Clinical Laboratory Standards (NCCLS) phenotypic ... micro dilution assay and E test ESBL strip are time consuming ...

Article: Comparison of double disc and three dimensional methods to screen ...
Indian Journal of Medical Microbiology;  April 1, 2006 ;     700+ words ... ... An attempt was made to study ESBL production among Enterobacteriaceae ... antibiotics and detection of ESBL producers by double disc synergy ... the National Committee for Clinical Laboratory Standards (NCCLS).[5 ... Tests for determination of ESBL activity Double disk synergy ...

Article: Multiplex PCR for bla^sub CTX-M^ & bla^sub SHV^ in the ...
Indian Journal of Medical Research;  September 1, 2008 ;     700+ words ... ... extended spectrum beta lactamase (ESBL) production in Gram-negative ... susceptibility testing and screening for ESBL enzyme production: Antimicrobial ... diffusion test5 according to the Clinical Laboratory Standards Institute (CLSI ... pneumoniae ATCC 700603 as an ESBL-positive control ...

Article: Detection of ESBL bacteria from clinical specimens: ...
British Journal of Biomedical Science;  October 1, 2008 ;     700+ words ... ... extended-spectrum β-lactamase (ESBL)-producing bacteria are needed to guide ... testing procedures and HPA/BSAC guidance on ESBL detection. The CCA medium was also evaluated for ESBL faecal carriage from patients on ICU and the ...

Article: Occurrence of ESBL & Amp-C [beta]-lactamases & ...
Indian Journal of Medical Research;  January 1, 2008 ;     700+ words ... ... was undertaken to know the occurrence of ESBL and AmpC producing strains and their antibiotic ... HDRU). HDRU were further tested for ESBL and AmpC phenotypes. Results: Uropathogens ... consecutive HDRU isolates were tested for ESBL production and 36.5 per cent were found ...

Article: Treatment of Bacteremia Due to ESBL-Producing ...
Infectious Disease Alert;  August 1, 2004 ;     700+ words ...Treatment of Bacteremia Due to ESBL-Producing Klebsiella pneumoniae Abstract ... choice for therapy of bacteremia due to ESBL-producing Klebsiella pneumoniae. Source ... 19%) of the episodes were due to an ESBL-producing K. pneumoniae; 20 (24 ...

Article: ESBL- From petri dish to the patient.(Special Article)
Indian Journal of Medical Microbiology;  January 1, 2006 ;     700+ words ... ... of extended spectrum beta-lactamase (ESBL) producing strains of Enterobacteriaceae ... community based patients. Infections due to ESBL producers range from uncomplicated urinary ... clinical questions in the management of ESBL producing organisms. The article emphasises ...

Article: Extended spectrum beta lactamase (ESBL) producing ...
Indian Journal of Medical Microbiology;  July 1, 2008 ;     700+ words ... ... monobactams.[sup] [1] Production of ESBL enzymes is either chromosomally mediated ... mediated.[sup] [2] The majority of ESBL producing strains are Klebsiella pneumoniae ... This study was carried out to find out ESBL producing E.coli and K.pneumoniae in ...

 
 
Newsweek Harper's Magazine The Washington Post Chicago Tribune Crain's Chicago Business PRNewswire Pediatric News The Nation Advertising Age The Economist (US) A FREE trial gives you access to over 80 million articles! Access over 6,500 publications with a FREE trial!

Find articles, research, and archives