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Article: Body Temperature-Related Structural Transitions of Monotremal and Human Hemoglobin
- Article from:
- Biophysical Journal
- Article date:
- October 15, 2006
- Author:
CopyrightCopyright Biophysical Society Oct 15, 2006. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
In this study, temperature-related structural changes were investigated in human, duck-billed platypus (Ornithorhynchus anatinus, body temperature 7^sub b^ = 31-33°C), and echidna (Tachyglossus aculeatus, body temperature T^sub b^ = 32-33°C) hemoglobin using circular dichroism spectroscopy and dynamic light scattering. The average hydrodynamic radius (R^sub h^) and fractional (normalized) change in the ellipticity (F^sub obs^) at 222 ± 2 nm of hemoglobin were measured. The temperature was varied stepwise from 25°C to 45°C. The existence of a structural transition of human hemoglobin at the critical temperature T^sub c^ between 36-37°C was previously shown by micropipette ...