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Article: Binding of T-Antigen Disaccharides to Artocarpus hirsuta Lectin and Jacalin are Energetically Different
- Article from:
- Photochemistry and Photobiology
- Article date:
- September 1, 2006
- Author:
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Copyright informationCopyright American Society for Photobiology Sep/Oct 2006. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
The thermodynamics of binding of Me-α-GalNAc, Gal-β-1-3GalNAc-α-O-Me (T-antigen-α), Gal-β-1-3GalNAc and Gal-α-1-6Glc (mellibiose) to Artocarpus hirsuta lectin was studied using fluorescence spectroscopy. The binding affinities of the saccharides are in the order Gal-β-1-3GalNAc-α-O-Me > Me-α-GalNAc > Me-α-Gal > Gal-β-1-3GalNAc > Gal-α-1-6Glc. The binding affinities were comparable to those for jacalin. However, binding of the saccharides to the A. hirsuta lectin was not affected as strongly by temperature as observed in jacalin and the trend was found to be reversed. Values for ΔH and ΔS were found to ...
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