|
|
Article: How to produce & characterize transgenic plants
- Article from:
- The American Biology Teacher
- Article date:
- April 1, 2002
- Author:
CopyrightCopyright National Association of Biology Teachers Apr 2002. Provided by ProQuest LLC. (Hide copyright information)
|
A simple and inexpensive protocol to produce and characterize transgenic plants for undergraduate students has been developed. Leaf tissue explants from greenhouse or in vitro-grown petunia or tobacco plants are incubated on a tissue culture medium to induce cell division and expansion. Selected explants are infected with Agrobacterium vir-helper strain EHA105 containing T-DNA vector pYI-mas. Vector pYI-mas contains three genes for the synthesis of novel carbon and nitrogen compounds called the mannityl opines and the plant selec- table marker gene conferring antibiotic resistance to the transformed plant cells. Ninety-seven percent of the explants infected with EHA105 (pYI-mas) gave rise ...