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Article: Determination of the Antihypertensive Peptide LHLPLP in Fermented Milk by High-Performance Liquid Chromatography-Mass Spectrometry
- Article from:
- Journal of Dairy Science
- Article date:
- December 1, 2006
- Author:
CopyrightCopyright American Dairy Science Association Dec 2006. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
Among different lactic acid bacteria isolated from raw milk, 4 Enterococcus faecalis strains have stood out as producers of fermented milk with potent antihypertensive activity. The peptide β-casein f(133-138), LHLPLP, was identified as one of the major peptides responsible for the activity of these fermented milk products. A simple method was developed to quantify this peptide in fermented milk using high-performance liquid chromatography coupled in line with mass spectrometry. This procedure does not require any previous sample fractionation or extraction, and direct analysis of the water-soluble extract obtained from the fermented milk can be performed. Validation studies ...