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Article: RNAprotect Saliva: An Optimal Room-Temperature Stabilization Reagent for the Salivary Transcriptome
- Article from:
- Clinical Chemistry
- Article date:
- December 1, 2006
- Author:
CopyrightCopyright American Association for Clinical Chemistry Dec 2006. Provided by ProQuest LLC. (Hide copyright information)
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To the Editor:
Quantitative analysis can now be performed on a panel of human salivary mRNAs identified as potential markers for oral cancer (1, 2). Translational and clinical applications of salivary transcriptome diagnostics require RNA degradation in saliva to be stopped at the time of collection and until analysis, preferably with room-temperature-compliant stabilization reagents.
We compared 3 RNA stabilizing reagents for their abilities to stabilize salivary RNA at room temperature: SUPERase.In(TM) RNase Inhibitor (SI), RNALater® (RL), and RNA protect® Saliva Reagent (RPS). We incubated saliva samples with these reagents and control saliva samples with no Stabilization reagent for up ...