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Article: Accelerated detection of extended-spectrum [beta]-lactamases in clinical isolates of Enterobacteriaceae
- Article from:
- British Journal of Biomedical Science
- Article date:
- October 1, 2006
- Author:
CopyrightCopyright Step Communications Ltd. 2006. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
A prospective study is carried out to evaluate the performance of a protocol for the accelerated detection of extended-spectrum β-lactamases (ESBLs) in clinical isolates of Escherichia coli, Klebsiella pneumoniae and other Gram-negative bacteria. A modified double-disc test (MDDT) is incorporated in a Gram-negative template for routine susceptibility testing. The MDDT identified accurately ESBLs in all isolates subsequently confirmed as ESBL-producers by the standard Clinical Laboratory Standards Institute (CLSI) combined disc method. Of 1213 isolates tested, 98 (8%) were positive for ESBLs by MDDT and 95 (7.8%) were positive by the CLSI method. ESBLs were detected in 48 ...