Articles > Journals > Applied Sciences journals > Biophysical Journal articles > January 2007

Article: The Gas-Phase Absorption Spectrum of a Neutral GFP Model Chromophore

Article from:
Biophysical Journal
Article date:
January 1, 2007
Author:
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ABSTRACT

We have studied the gas-phase absorption properties of the green fluorescent protein (GFP) chromophore in its neutral (protonated) charge state in a heavy-ion storage ring. To accomplish this we synthesized a new molecular chromophore with a charged NH^sub 3^ group attached to a neutral model chromophore of GFP. The gas-phase absorption cross section of this chromophore molecule as a function of the wavelength is compared to the well-known absorption profile of GFP. The chromophore has a maximum absorption at 415 ± 5 nm. When corrected for the presence of the charged group attached to the GFP model chromophore, the unperturbed neutral chromophore is predicted to have an absorption ...

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