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Article: Experimental Hyperhomocysteinemia Reduces Bone Quality in Rats
- Article from:
- Clinical Chemistry
- Article date:
- August 1, 2007
- Author:
CopyrightCopyright American Association for Clinical Chemistry Aug 2007. Provided by ProQuest LLC. (Hide copyright information)
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Background: Recently, hyperhomocysteinemia (HHCY) has been suggested as a new risk factor for osteoporosis. This study investigated if HHCY is a causal osteoporotic factor in vivo.
Methods: We used 3 groups of rats: a control group (n = 20), a moderate HHCY group (induced by a 2.4% methionine-enriched diet, n = 10), and an intermediate HHCY group (induced by a 2% homocystine-enriched diet, n = 10). We measured bone fragility [maximum force of an axial compression test (F^sub max^)], bone area as percentage of total area (BAr/TAr, histomorphometry), and biochemical bone turnover markers [osteocalcin (OC) and collagen I C-terminal crosslaps (CTx)].
Results: Compared with controls, 3 months of ...