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Article: Interaction of Fusarium solani Lectin with Monosaccharides and Oligosaccharides: A Fluorometric Study
- Article from:
- Photochemistry and Photobiology
- Article date:
- July 1, 2007
- Author:
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Copyright informationCopyright American Society for Photobiology Jul/Aug 2007. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
The intrinsic fluorescence intensity of Fusarium solani lectin was quenched upon binding to mono- and oligosaccharides without any change in the emission maximum and it was used to determine association constants for several sugars and glycans. The lectin interacted very poorly with monosaccharides but well with disaccharides (T-antigen and LacNAc) with a distinction between β1 [arrow right] 4 and β1 [arrow right] 3 linkages. Among the monosaccharides, the interaction was observed only with Gal/GalNAc derivatives and not with Glc/Man derivatives. Thermodynamic studies revealed that the binding of the lectin with all the saccharides is enthalpically driven and exothermic ...
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