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Article: In vitro host range of the Hz-1 nonoccluded virus in insect cell lines
- Article from:
- In Vitro Cellular & Developmental Biology
- Article date:
- May 1, 2007
- Author:
CopyrightCopyright Society for In Vitro Biology May/Jun 2007. Provided by ProQuest LLC. (Hide copyright information)
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Abstract A total of 13 insect cell lines spanning 4 orders (Lepidoptera, Coleoptera, Diptera, and Homoptera) were tested for their ability to replicate the nonoccluded virus Hz-1. Only the Lepidopteran cell lines supported replication of the virus with TN-CL1 and BCIRL-HZ-AM1 producing the highest titers of 2.4Ã10^sup 8^ tissue culture infective dose (TCID)^sub 50^/ml and 2.0Ã10^sup 8^ TCID^sub 50^/ml, respectively. A codling moth cell line (CP-169) was the only Lepidopteran cell line that did not replicate the virus and transfection of this cell line with Hz-1 DNA failed to replicate the virus. Also, transfection with DNA from a recombinant baculovirus carrying the red fluorescent ...
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