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Article: Bid Is Required in NPe6-PDT-induced Apoptosis
- Article from:
- Photochemistry and Photobiology
- Article date:
- January 1, 2008
- Author:
CopyrightCopyright American Society for Photobiology Jan/Feb 2008. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
Photodynamic therapy (PDT) employing photosensiter N-aspartyl chlorin e6 (NPe6) can induce lysosome disruption and initiate the intrinsic apoptotic pathway. Yet the precise signal transduction pathway remains poorly understood. In this study, we have investigated the molecular mechanism in NPe6-PDT-induced apoptosis in human lung adenocarcinoma cells (ASTC-a-1). A recombinant fluorescence resonance energy transfer (FRET) Bid probe was utilized to determine the kinetics of Bid cleavage. The results show that cleavage of the Bid-FRET probe occurred 150 ± 5 min after NPe6-PDT treatment, and this process lasted for 45 ± 5 min. The Bid cleavage coincided with a translocation of tBid ...