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Article: Local Anesthetics Have Different Mechanisms and Sites of Action at Recombinant 5-HT^sub 3^ Receptors
- Article from:
- Regional Anesthesia and Pain Medicine
- Article date:
- November 1, 2007
- Author:
CopyrightCopyright Churchill Livingstone Inc., Medical Publishers Nov/Dec 2007. Provided by ProQuest LLC. (Hide copyright information)
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Background and Objectives: In addition to their blockade of voltage-dependent sodium channels, the action of local anesthetics at 5-hydroxytryptamine-3 (5-HT^sub 3^) receptors may be clinically relevant. Because local anesthetics have different clinical properties, we have tested the hypothesis that differences in interactions at the 5-HT^sub 3^ receptor may be clinically relevant by investigating the effects of 4 local anesthetics on recombinant wild-type and 4 mutant 5-HT^sub 3A^ receptors.
Methods: The cRNAs from human wild-type and 4 mutant 5-HT^sub 3A^ subunit clones were synthesized in vitro and expressed in Xenopus oocytes. Four mutant receptors were obtained by site-directed ...