|
|
Effect of Metabolic Inhibition on Couplon Behavior in Rabbit Ventricular Myocytes
- Article from:
-
Biophysical Journal
- Article date:
-
March 1, 2008
- Author:
- Chantawansri, Chana; Huynh, Nhi; Yamanaka, Jun; Garfinkel, Alan; Lamp, Scott T; Inoue, Masashi; Bridge, John H B; Goldhaber, Joshua I
|
Copyright informationCopyright Biophysical Society Mar 1, 2008. Provided by ProQuest LLC. (Hide copyright information)
|
ABSTRACT
We investigated the effect of combined inhibition of oxidative and glycolytic metabolism on L-type Ca^sup 2+^ channels (LCCs) and Ca^sup 2+^ spikes in isolated patch-clamped rabbit ventricular myocytes. Metabolic inhibition (MI) reduced LCC open probability, increased null probability, increased first latency, and decreased open time but left conductance unchanged. These results explain the reduction in macroscopic Ca^sup 2+^ current observed during MI. MI also produced a gradual reduction in action potential duration at 90% repolarization (APD^sub 90^), a clear decline in spike probability, and an increase in spike latency and variance. These effects are consistent with the ...