The Interfacial Lipid Binding Site on the Potassium Channel KcsA Is...

Biophysical Journal;  December 1, 2005 ;   Marius, Phedra; Alvis, Simon J; East, J Malcolm; Lee, Anthony G;   787 words ......the absence of anionic lipid, the nonannular sites are occupied by...know how strongly anionic lipid binds to the nonannular sites; the fact that...occupancy of the nonannular sites by anionic lipid in the native...

Theory of tunable pH-sensitive vesicles of anionic and cationic lipids or...

Biophysical Journal;  April 1, 2001 ;   Li, Xiao-jun; Schick, M;   787 words ......is applied to a system of ionizable anionic lipid and permanently charged cationic lipid...nonlamellar-forming lipid stabilized by an anionic lipid would serve equally well for preparing...They use a vesicle with an ionizable anionic lipid, cholesteryl hemisuccinate (CHEMS...

Intramembrane electrostatic interactions destabilize lipid vesicles

Biophysical Journal;  October 1, 2002 ;   Shoemaker, Scott D; Vanderlick, T Kyle;   787 words ......solutions. We show that inclusion of anionic lipid does not appreciably alter the areal...vesicle rupture decreases with increasing anionic lipid fraction and is a function of electrolyte...vesicles with 30% charged (i.e., unbound) anionic lipid, we measured critical tension ...

Investigations into the Membrane Interactions of m-Calpain Domain V

Biophysical Journal;  April 1, 2005 ;   Dennison, Sarah R; Dante, Silvia; Hauß, Thomas; Brandenburg, Klaus; Et al;   787 words ......concentration range and did not alter when the anionic lipid composition of these vesicles was varied...that correlated strongly with their anionic lipid content (maximal at 4 mN m^sup -1...needed for structural stabilization anionic lipid was required for membrane penetration...

A Quantitative Model for the All-or-None Permeabilization of Phospholipid...

Biophysical Journal;  March 1, 2008 ;   Gregory, Sonia M; Cavenaugh, Allison; Journigan, Velvet; Pokorny, Antje; Almeida, Paulo F F;   787 words ......and, at constant concentration, with the fraction of the anionic lipid phosphatidylglycerol in neutral, phosphatidylcholine membranes...decreases by orders of magnitude with increasing content of anionic lipid. A simple, exact model fits all experimental kinetic data...

Cytochrome c Adsorption to Supported, Anionic Lipid Bilayers Studied via...

Biophysical Journal;  November 1, 2004 ;   Choi, Eugene J; Dimitriadis, Emilios K;   787 words ......associated protein cytochrome c to anionic lipid bilayers of dioleoyl phosphatidyl-glycerol...c either adsorbs to the surface of anionic lipid membranes or inserts into the membrane...inserts into bilayers composed of the anionic lipid dioleoyl phosphatidylglycerol (DOPG...

A Molecular View on the Interaction of the Trojan Peptide Penetratin with...

Biophysical Journal;  July 1, 2004 ;   Binder, Hans; Lindblom, Göran;   787 words ......presence of zwitterionic dimyristoylphosphatidylcholine and of anionic lipid membranes composed of dioleoylphosphatidylcholine and dioleoylphosphatidylglycerol...polar part of the membranes and the effective compensation of anionic lipid charges. INTRODUCTION Trojan peptides are able to ...

Polylysine-induced (2)H NMR-observable domains in...

Biophysical Journal;  December 1, 2001 ;   Franzin, Carla M; Macdonald, Peter M;   787 words ......conditions of low ionic strength and for cases where the global anionic lipid charge was in excess over the global cationic polypeptide...under physiological conditions of ionic strength, pH, and anionic lipid bilayer content, and in the absence of other, e.g., hydrophobic...

Domain-Induced Activation of Human Phospholipase A^sub 2^ Type IIA: Local...

Biophysical Journal;  May 1, 2006 ;   Leidy, Chad; Linderoth, Lars; Andresen, Thomas L; Mouritsen, Ole G; Et al;   787 words ......to the extracellular face. However, anionic lipid contents in native cells appear lower...perturbed native membranes with low global anionic lipid contents. The results also show that...and hydrolyze both zwitterionic and anionic lipid membranes, and do not exhibit the specificity...

Lipid-Protein Interactions of Integral Membrane Proteins: A Comparative...

Biophysical Journal;  December 1, 2004 ;   Deol, Sundeep S; Bond, Peter J; Domene, Carmen; Sansom, Mark S P;   787 words ......Bulieris et al., 2003). For example, in the case of the K channel KcsA, acidic phospholipids appear to bind to specific (nonannular) sites at which they play a role in refolding and possibly in function (Valiyaveetil et al., 2002; Demmers et al., 2003; Alvis...

Charge-dependent translocation of the Trojan peptide penetratin across...

Biophysical Journal;  August 1, 2003 ;   Binder, Hans; Lindblom, Goran;   787 words ......dioleoylphosphatidylcholine/dioleoylphoshatidylglycerol (DOPC/DOPG) unilamellar vesicles as a function of the molar fraction of anionic lipid, X^sub PG^, by means of isothermal titration calorimetry. The work was aimed at getting a better understanding of factors...

Antimicrobial Peptide-Lipid Binding Interactions and Binding Selectivity

Biophysical Journal;  May 15, 2007 ;   Lad, Mitaben D; Birembaut, Fabrice; Clifton, Luke A; Frazier, Richard A; Et al;   787 words ......melittin, magainin II, and cecropin P1. As expected, all three cationic peptides were shown to interact more strongly with the anionic lipid, 1,2 dihexadecanoyl-sn-glycerol-3-(phosphor-rac-(1-glycerol)) (DPPG), compared to the zwitterionic lipid, 1,2 dihexadecanoyl-sn-glyc...

Europium III binding and the reorientation of magnetically aligned...

Biophysical Journal;  July 1, 2001 ;   Crowell, Kevin J; Macdonald, Peter M;   787 words ......composed of 1,2-dimyristoyl-sn-glycero-3phosphocholine (DMPC) plus 1,2-dihexanoyl-sn-glycero-3-phosphocholine plus either the anionic lipid 1,2-dimyristoyl-sn3-phosphoglycerol, or the cationic lipid 1,2-dimyristoyl-3-trimethyl ammonium propane. Alignment of the...

Formation of Three-Dimensional Structures in Supported Lipid Bilayers

Biophysical Journal;  May 15, 2007 ;   Cambrea, Lee R; Hovis, Jennifer S;   787 words ......an asymmetry in ionic strength. The structures that form depend on the asymmetry in the ionic strength and the amount of anionic lipid. Other factors that may be of importance in the creation of the structures, expansion forces, osmotic forces, and the bilayer-suppo...

Saturated anionic phospholipids enhance transdermal transport by...

Biophysical Journal;  October 1, 2002 ;   Sen, Arindam; Zhao, Ya-li; Hui, Sek Wen;   787 words ......dioleoylphosphatidylserine (DOPS), and dioleoylphosphatidylglycerol (DOPG) were used to test factors affecting the potency of anionic lipid transport enhancers. DMPS with saturated acyl chains was found to be a much more potent transport enhancer than those with...