|
|
Article: Immunodetection of Glycosylated NT-proBNP Circulating in Human Blood
- Article from:
- Clinical Chemistry
- Article date:
- May 1, 2008
- Author:
CopyrightCopyright American Association for Clinical Chemistry May 2008. Provided by ProQuest LLC. (Hide copyright information)
|
BACKGROUND: Brain natriuretic peptide (BNP) or NT-proBNP (N-terminal fragment of BNP precursor) measurements are recommended as aids in diagnosis and prognosis of patients with heart failure. Recently it has been shown that proBNP is O-glycosylated in human blood. The goal of this study was to map sites on the NT-proBNP molecule that should be recognized by antibodies used in optimal NT-proBNP assays.
METHODS: We analyzed endogenous NT-proBNP by several immunochemical methods using a broad panel of monoclonal antibodies specific to different epitopes of the NT-proBNP molecule.
RESULTS: Treatment of endogenous NT-proBNP by a mixture of glycosidases resulted in significant improvement of the ...