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Distinct Functions of MLH3 at Recombination Hot Spots in the Mouse
- Article from:
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Genetics
- Article date:
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April 1, 2008
- Author:
- Svetlanov, Anton; Baudat, Frederic; Cohen, Paula E; de Massy, Bernard
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Copyright informationCopyright Genetics Society of America Apr 2008. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
The four mammalian MutL homologs (MLH1, MLH3, PMS1, and PMS2) participate in a variety of events, including postreplicative DNA repair, prevention of homeologous recombination, and crossover formation during meiosis. In this latter role, MLH1-MLH3 heterodimers predominate and are essential for prophase I progression. Previous studies demonstrated that mice lacking Mlh1 exhibit a 90% reduction in crossing over at the Psmb9 hot spot while noncrossovers, which do not result in exchange of flanking markers but arise from the same double-strand break event, are unaffected. Using a PCR-based strategy that allows for detailed analysis of crossovers and noncrossovers, we show here that ...