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Triple immunofluorescence labeling of atherosclerotic plaque components in apoE/LDLR^sup -/-^ mice
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Folia Histochemica et Cytobiologica
- Article date:
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April 1, 2008
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Copyright informationCopyright Folia Histochemica et Cytobiologica 2008. Provided by ProQuest LLC. (Hide copyright information)
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Abstract: This paper presents a simple and reliable method of triple immunofluorescence staining that allows simultaneous detection of various cell types present in atherosclerotic plaque of apolipoprotein E and LDL receptor-double knockout (apoE/LDLR ^sup -/-^) mice. We used combined direct and indirect procedures applying commercially available primary antibodies raised in different species to detect smooth muscle cells (Cy3-conjugated mouse anti-smooth muscle actin, SMA), macrophages (rat anti-CD68) and T lymphocytes (rabbit anti-CD3). Fixation of the material in acetone and modified incubation protocol employing nonfat dry milk in preincubation and incubation media significantly ...
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