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Osmotic stress induces terminal differentiation in cultured normal human epidermal keratinocytes
- Article from:
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In Vitro Cellular & Developmental Biology
- Article date:
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May 1, 2008
- Author:
- Mammone, Thomas; Ingrassia, Michael; Goyarts, Earl
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Copyright informationCopyright Society for In Vitro Biology May/Jun 2008. Provided by ProQuest LLC. (Hide copyright information)
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Abstract The signals for epidermal differentiation and barrier formation are largely unknown. One possible signal is dehydration or osmotic stress. To test this hypothesis, we investigated the effects of osmotic stress on markers of differentiation of normal human keratinocytes in culture. Hyperosmotic stress treatment of normal human keratinocyte cultures by elevated sorbitol concentrations was observed to induce markers of terminal differentiation. Sorbitol was added to keratinocyte media at 50, 100, 200, and 300 mM final concentration. These concentrations of sorbitol induce a dehydration effect or osmotic stress on the keratinocytes. These sorbitol treatments increased the levels of ...
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UC San Diego Team Determines Cellular Stress Within Body Is Critical...
Ascribe Higher Education News Service;
July 6, 2004 ;
705 words
......particular type of cellular stress called osmotic stress is of critical importance to cell growth...cell proliferation under conditions of osmotic stress and is also necessary for the body's immune response to invading pathogens. Osmotic stress occurs when the concentration of ...
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Integration of wounding and osmotic stress signals determines the...
Plant Physiology;
July 1, 2003 ;
Denekamp, Marten; Smeekens, Sjef C;
787 words
......named AtM4, are rapidly induced by osmotic stress or abscisic acid (ABA) treatment. Reporter...untranslated region is essential for the osmotic stress and wounding induced expression of...signals derived from both wounding and osmotic stress. Abscisic acid (ABA) is the major plant...
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Requirement for the Polarisome and Formin Function in Ssk2p-Mediated Actin...
Genetics;
April 1, 2007 ;
Bettinger, Blaine T; Clark, Michael G; Amberg, David C;
787 words
...ABSTRACT Osmotic stress induces activation of an adaptive...the actin cytoskeleton following osmotic stress. In this study, we have employed...is the primary mediator of the osmotic stress response (reviewed by Sheikh-Hamad...
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Cyclic AMP and Aquaporin Trafficking
Plant Physiology;
August 1, 2004 ;
Minorsky, Peter V;
397 words
......target membrane under conditions of osmotic stress. Indeed, in animals, regulatory...distribution of McTIPl;2 is altered by osmotic stress. Differences in the response of McTIPl;2 to salt and osmotic stress suggest that the ionic component...
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Identification of a Novel Gene Family Involved in Osmotic Stress Response...
Genetics;
November 1, 2006 ;
Wheeler, Jeanna M; Thomas, James H;
787 words
......accumulates glycerol in response to osmotic stress, activated primarily by MAP kinase...different from yeast to mount a response to osmotic stress. ALL living organisms have mechanisms...taurine (BURG et al. 1997). Adaptation to osmotic stress has been studied extensively in yeast...
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Proteomic Identification of Annexins, Calcium-Dependent Membrane Binding...
Plant Cell;
June 1, 2004 ;
Lee, Sumin; Lee, Eun Jung; Yang, Eun Ju; Lee, Ji Eun; Et al;
787 words
......annAt4, displayed hypersensitivity to osmotic stress and abscisic acid (ABA) during germination...and AnnAt4 play important roles in osmotic stress and ABA signaling in a Ca^sup 2+^-dependent...cytosol, which in turn induce ionic and osmotic stress in plants, respectively. ...
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The effect of water on the rate of conformational change in protein...
Biophysical Journal;
November 1, 2001 ;
Goldbeck, Robert A; Paquette, Sarah J; Kliger, David S;
787 words
......photodissociation of aqueous HbCO complex under osmotic stress induced by the nonbinding cosolute...constants and amplitudes as a function of osmotic stress and viscosity. Increases in time constants...for small cosolutes showed that both osmotic stress and solvent viscosity are ...
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Osmotic induction of stress-responsive gene expression in the lobster...
The Biological Bulletin;
December 1, 2002 ;
Spees, Jeffrey L. Chang, Sharon A. Snyder, Mark J. Chang, Ernest S.;
787 words
......Homarus americanus, encounters osmotic stress throughout its life cycle. To understand the molecular basis of osmotic stress responses in vivo, we used homologous...polyubiquitin during hypo- and hyper-osmotic stress in abdominal muscle and hepatopancreas...
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An Arabidopsis GSK3/shaggy-like gene that complements yeast salt...
Plant Physiology;
April 1, 1999 ;
Piao, Hai Lan; Pih, Kyeong Tae; Lim, Jeong Hwa; Kang, Shin Gene; Et al;
787 words
......suggest that AtGSK1 is involved in the osmotic stress response in Arabidopsis. Abbreviations...changes that occur upon exposure to osmotic stress (Walton, 1980; DuPont, 1992; Bohnert...of different mechanisms to cope with osmotic stress. Responses are especially well understood...
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Osmotic and curvature stress affect PEG-induced fusion of lipid vesicles...
Biophysical Journal;
April 1, 2002 ;
Malinin, Vladimir S; Frederik, Peter; Lentz, Barry R;
787 words
......close contact was not influenced by osmotic stress or membrane curvature, although pore...same part of the fusion process as osmotic stress. We interpret this result to suggest...concentrations of 20-40 wt%, causing osmotic stress of cells or vesicles when PEG added...
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Novel Regulation of Aquaporins during Osmotic Stress1
Plant Physiology;
August 1, 2004 ;
Vera-Estrella, Rosario; Barkla, Bronwyn J; Bohnert, Hans J; Pantoja, Omar;
787 words
......regulation and redistribution in response to osmotic stress was investigated. Ice plant (Mesembryanthemum...maintain cellular osmolarity under osmotic-stress conditions. It seems intuitively obvious...which accompanies the perception of osmotic stress, have been shown to regulate the ...
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Osmotic stress tolerance of transgenic tobacco expressing a gene encoding...
Plant Physiology;
February 1, 2003 ;
Tamura, Takashi; Hara, Kojiro; Yamaguchi, Yube; Koizumi, Nozomu; Sano, Hiroshi;
787 words
...Osmotic Stress Tolerance of Transgenic Tobacco Expressing...systems usually requires three steps: osmotic stress recognition, signal transduction...pressure. In Escherichia coli and yeast, osmotic stress is detected by the osmosensors EnvZ...
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Extreme dark cytotoxicity of nile blue A in normal human fibroblasts
Photochemistry and Photobiology;
November 1, 2001 ;
Tong, Zhimin; Singh, Gurmit; Rainbow, Andrew J;
787 words
......Dark Cytotoxicity of Nile Blue A in Normal Human Fibroblasts(para) ABSTRACT Early reports...NBA-mediated photodynamic treatment in normal human cells are lacking. In the current study...examined the cytotoxicity of NBA in normal human fibroblasts, spontaneously immortalized...
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The role of hydration on the mechanism of allosteric regulation: In situ...
Biophysical Journal;
January 1, 2003 ;
Salvay, Andres G; Grigera, J Raul; Colombo, Marcio F;
787 words
......water-accessible surface area, isobaric osmotic stress experiments in aqueous cosolutions...agree with the ones determined by osmotic stress in aqueous cosolutions, from the linkage...Parsegian et al., 1995). The proposal of osmotic stress strategy has greatly contributed to...
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Arabidopsis TARGET OF RAPAMYCIN Interacts with RAPTOR, Which Regulates the...
Plant Cell;
February 1, 2006 ;
Mahfouz, Magdy M; Kim, Sunghan; Delauney, Ashton J; Verma, Desh Pal S;
787 words
......kinase (S6K) in response to osmotic stress. RAPTOR1 also interacts in...S6K1 activity was sensitive to osmotic stress, while PDK1 activity was not...However, S6K1 sensitivity to osmotic stress was relieved by co-overexpression...
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