Abstract In vitro production of the obligate intracellular bacterium, Wolhachia pipientis, is essential to its manipulation as a genetic tool to spread transgenes within vector populations. We have adapted the Wolbachia-infected Aa23 Aedes albopictus mosquito cell line to Eagle's minimal medium, supplemented with nonessential amino acids, glutamine, and 20% fetal bovine serum. When plated at low densities, Aa23E cells grew as patchy monolayers, comprised of non-contiguous clusters of cells that gave rise to solid clumps of tightly adherent cells. Multicellular clumps eventually detached from the substrate and floated freely in the medium. Removal of Wolhachia by treatment with tetracycline ...

Related newspaper, magazine, and journal articles:

;

See all results. Or, try our Advanced Search.

We're searching over:

• 60 million articles
• 3,500 publications