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Electrophysiological and immunocytochemical characterization of DRG neurons on an organosilane surface in serum-free medium

Abstract We are attempting to recreate a stretch reflex circuit on a patterned Rio-MEMS (bio-microelcctromeehanical systems) chip with deflecting micro-cantilevers. The first steps to recreate this system is to be able to grow individual components of the circuit (sensory neuron, motoneuron, skeletal muscle, and muscle spindle) on a patternable, synthetic substrate coating the MEMS device. Sensory neurons represent the afferent portion of the stretch reflex are and also play a significant role in transmitting the signal from the muscle spindle to the spinal cord motoneurons. We have utilized a synthetic silane substrate N-1[3-(trimethoxysilyl) propyl) diethylenetriamine (DETA) on which to ...

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