Comparison of potassium hydroxide mount and mycological culture with histopathologic examination using periodic acid-Schiff staining of the nail clippings in the diagnosis of onychomycosis

Introduction

Onychomycosis is a common problem in clinical practice, accounting for up to 50% of all nail diseases. [1] It is caused by a variety of fungal agents and presents in several clinical forms. It can mimic several diseases in the field of dermatology and internal medicine. It is necessary to diagnose the infection with some laboratory evidences before treating them with antifungal drugs, whose duration of treatment is long and may have some serious side effects. Currently available routine laboratory methods are direct microscopy with potassium hydroxide mount (KOH) and mycological culture. These traditional methods give inconsistent results, and the results may be altered ...

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