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How Cells Tiptoe on Adhesive Surfaces before Sticking
- Article from:
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Biophysical Journal
- Article date:
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May 15, 2008
- Author:
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Copyright informationCopyright Biophysical Society May 15, 2008. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
Cell membranes are studded with protrusions that were thoroughly analyzed with electron microscopy. However, the nanometer-scale three-dimensional motions generated by cell membranes to fit the topography of foreign surfaces and initiate adhesion remain poorly understood. Here, we describe the dynamics of surface deformations displayed by monocytic cells bumping against fibronectin-coated surfaces. We observed membrane undulations with typically 5 nm amplitude and 5-10 s lifetime. Cell membranes behaved as independent units of micrometer size. Cells detected the presence of foreign surfaces at 50 nm separation, resulting in time-dependent amplification of membrane undulations. ...
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