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Article: Confirming the Revised C-Terminal Domain of the MscL Crystal Structure
- Article from:
- Biophysical Journal
- Article date:
- June 15, 2008
- Author:
CopyrightCopyright Biophysical Society Jun 15, 2008. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
The structure of the C-terminal domain of the mechanosensitive channel of large conductance (MscL) has generated significant controversy. As a result, several structures have been proposed for this region: the original crystal structure (1MSL) of the Mycobacterium tuberculosis homolog (Tb), a model of the Escherichia coli homolog, and, most recently, a revised crystal structure of Tb-MscL (2OAR). To understand which of these structures represents a physiological conformation, we measured the impact of mutations to the C-terminal domain on the thermal stability of Tb-MscL using circular dichroism and performed molecular dynamics simulations of the original and the revised crystal ...