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Acute Administration of Cefepime Lowers L-Carnitine Concentrations in Early Lactation Stage Rat Milk1-3

Abstract

Our study investigated the potential for important in vivo drug-nutrient transport interactions at the lactating mammary gland using the L-carnitine transporter substrates, cefepime and L-carnitine, as proof-of-concept. On d 4 (n = 6/treatment) and d 10 (n = 6/treatment) of lactation, rats were administered cefepime (250 mg/h) or saline by continuous i.v. infusion (4 h). Serum and milk L-carnitine and cefepime concentrations were quantified by HPLC-UV. In whole mammary gland, organic cation/carnitine transporter (OCTN)1, OCTN2, OCTN3, amino acid transporter B^sup 0,+^ (ATB^sup 0,+^), and L-carnitine transporter 2 expression were determined by quantitative RT-PCR and by western blot ...

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