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Article: SCAMPs Highlight the Developing Cell Plate during Cytokinesis in Tobacco BY-2 Cells1[C][W][OA]
- Article from:
- Plant Physiology
- Article date:
- August 1, 2008
- Author:
CopyrightCopyright American Society of Plant Biologists Aug 2008. Provided by ProQuest LLC. (Hide copyright information)
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We previously demonstrated that rice (Oryza sativa) SECRETORY CARRIER MEMBRANE PROTEIN1 (OsSCAMP1)-yellow fluorescent protein in transgenic tobacco (Nicotiana tabacum) Bright Yellow-2 cells locates to the plasma membrane and to motile punctate structures, which represent the trans-Golgi network/early endosome and are tubular-vesicular in nature. Here, we now show that SCAMPs are diverted to the cell plate during cytokinesis dividing Bright Yellow-2 cells. As cells progress from metaphase to cytokinesis, punctate OsSCAMP1-labeled structures begin to collect in the future division plane. Together with the internalized endosomal marker FM4-64, they then become incorporated into the cell plate ...