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Functional Characterization of an Unusual Phytochelatin Synthase, LjPCS3, of Lotus japonicus1[W][OA]
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Plant Physiology
- Article date:
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September 1, 2008
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Copyright informationCopyright American Society of Plant Biologists Sep 2008. Provided by ProQuest LLC. (Hide copyright information)
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In plants and many other organisms, phytochelatin synthase (PCS) catalyzes the synthesis of phytochelatins from glutathione in the presence of certain metals and metalloids. We have used budding yeast (Saccharomyces cerevisiae) as a heterologous system to characterize two PCS proteins, LjPCS1 and LjPCS3, of the model legume Lotus japonicus. Initial experiments revealed that the metal tolerance of yeast cells in vivo depends on the concentrations of divalent cations in the growth medium. Detailed in vivo (intact cells) and in vitro (broken cells) assays of PCS activity were performed with yeast expressing the plant enzymes, and values of phytochelatin production for each metal tested were ...
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