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Gating of heteromeric retinal rod channels by cyclic AMP: Role of the C-terminal and pore domains

ABSTRACT Cyclic nucleotide-gated channels are tetramers composed of homologous a and beta subunits. C-terminal truncation mutants of the a and beta subunits of the retinal rod channel were expressed in Xenopus oocytes, and analyzed for cGMP- and cAMP-induced currents (single-channel records and macroscopic currents). When the a subunit truncated downstream of the cGMP-binding site (aD608stop) is co-injected with truncated beta subunits, the heteromeric channels present a drastic increase of cAMP sensitivity. A partial effect is observed with heteromeric aR656stop-containing channels, while aK665stop-containing channels behave like awt/lwt. The three truncated a subunits have wild-type ...

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