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Influence of pH upon Surface-enhanced Enzyme-catalyzed Luminol Chemiluminescence at Vicinity of Nanoscale-corrugated Gold and Silver Films

ABSTRACT

Au and Ag biochips were fabricated to investigate the influence of pH upon the chemiluminescence (CL) of luminol at vicinity of surface-adsorbed peroxidase. A nanoscaled-corrugation of the metal induces an enhancement of the luminol CL which is maximal in the pH range favoring peroxidase catalysis and greater for gold than for silver. This is the proof that, in the CL process, the reactions involving peroxidase are surface-enhanced near corrugated surfaces.

INTRODUCTION

Chemiluminescence (CL) is extensively studied as an important analytic technique in biotechnology and medical diagnostics due to its simplicity, rapidity and high sensitivity (1). Among the reactions producing CL, ...

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