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Effects of SERCA and PMCA Inhibitors on the Survival of Rat Cochlear Hair Cells during Ischemia in vitro
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Physiological Research
- Article date:
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July 1, 2008
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Copyright informationCopyright Institute of Physiology 2008. Provided by ProQuest LLC. (Hide copyright information)
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Summary
An important mechanism underlying cochlear hair cell (HC) susceptibility to hypoxia/ischemia is the influx of Ca^sup 2+^. Two main ATP-dependent mechanisms contribute to maintaining low Ca^sup 2+^ levels: uptake of Ca^sup 2+^ into intracellular stores via smooth endoplasmic reticulum calcium ATPase (SERCA) and extrusion of Ca^sup 2+^ via plasma membrane calcium ATPase (PMCA). The effects of the SERCA inhibitors thapsigargin (10 nM-10 µM) and cyclopiazonic acid (CPA; 10-50 µM) and of the PMCA blockers eosin (1.5-10 µM) and o-vanadate (1-5 mM) on inner and outer hair cells (IHCs/OHCs) were examined in normoxia and ischemia using an in vitro model of the newborn rat cochlea. ...
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