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Article: Molecular Epidemiology and Population Structure of Bovine Streptococcus uberis
- Article from:
- Journal of Dairy Science
- Article date:
- December 1, 2008
- Author:
CopyrightCopyright American Dairy Science Association Dec 2008. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
The molecular epidemiology and population structure of 30 bovine subclinical mastitis field isolates of Streptococcus uberis, collected from 6 Portuguese herds (among 12 farms screened) during 2002 and 2003, were examined by using pulsed-field gel electrophoresis (PFGE) for clustering of the isolates and multilocus sequence typing (MLST) to assess the relationship between PFGE patterns and to identify genetic lineages. The 30 isolates were clustered into 18 PFGE types, using a similarity cutoff of 80%, and 3 PFGE types accounted for almost half of the isolates (46.6%). These major types were herd specific, suggesting either cow-to-cow transmission or infection with isolates from ...