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Article: Automated Measurement of 25-OH Vitamin D^sub 3^ on the Roche Modular E170 Analyzer
- Article from:
- Clinical Chemistry
- Article date:
- December 1, 2008
- Author:
CopyrightCopyright American Association for Clinical Chemistry Dec 2008. Provided by ProQuest LLC. (Hide copyright information)
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BACKGROUND: The first commercial direct automated immunoassay specific for 25-OH vitamin D^sub 3^ (25-OH-D^sub 3^) was recently introduced for use on Roche Diagnostics immunoassay analyzers. We assessed the analytical performance of the Elecsys 25-OH-D^sub 3^ assay on a Roche Modular E 170 analyzer.
METHODS: The Elecsys 25-OH-D^sub 3^ assay is a direct electrochemiluminescence immunoassay for human serum or plasma. It is a competitive assay in which the binding protein of vitamin D is inactivated during incubation. The assay employs a polyclonal antibody directed against 25-OH vitamin D^sub 3^. We compared the 25-OH-D^sub 3^ assay to assays performed with RIA, HPLC, and liquid ...