Article: Indicator Amino Acid Oxidation Is Not Affected by Period of Adaptation to a Wide Range of Lysine Intake in Healthy Young Men1-3

Abstract

The number of days of adaptation to a specific amino acid intake required prior to the determination of amino acid requirements using the indicator amino acid oxidation method (IAAO) is still in debate. In this study, our objective was to determine whether adaptation for 8 h, 3 d, and 7 d to a wide range of lysine intakes had any effect on the oxidation of the indicator amino acid, L-[1-^sup 13^C]phenylalanine, to ^sup 13^CO^sub 2^ (F^sup 13^CO^sub 2^). Five healthy young men randomly received each of 4 levels of lysine (5, 20, 35, and 70 mg . kg^sup -1^ . d^sup -1^) along with an amino acid mixture to achieve a protein intake of 1.0 g . kg^sup -1^ . d^sup -1^ and energy intake of ...

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