Article: Glycation and phosphorylation of [alpha]-lactalbumin by dry heating: Effect on protein structure and physiological functions

ABSTRACT

α-Lactalbumin (α-LA) was glycated with maltopentaose (MP) through the Maillard reaction (MP-α-LA) and subsequently phosphorylated by dry heating in the presence of pyrophosphate to investigate its structure and physiological functions. Glycation occurred effectively, and the sugar content of α-LA increased by approximately 22.3% through the Maillard reaction. The phosphorylation of MP-α-LA was enhanced with an increase in the dry-heating time from 1 to 5 d, and the phosphorous content of MP-α-LA increased by approximately 1.01% by dry heating at pH 4.0 and 85°C for 5 d in the presence of pyrophosphate. The electrophoretic mobility of α-LA ...

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