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Article: Investigating unsaturated fat, monensin, or bromoethanesulfonate in continuous cultures retaining ruminal protozoa. I. Fermentation, biohydrogenation, and microbial protein synthesis1
- Article from:
- Journal of Dairy Science
- Article date:
- August 1, 2009
- Author:
CopyrightCopyright American Dairy Science Association Aug 2009. Provided by ProQuest LLC. (Hide copyright information)
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ABSTRACT
Methane is an end product of ruminal fermentation that is energetically wasteful and contributes to global climate change. Bromoethanesulfonate, animal-vegetable fat, and monensin were compared with a control treatment to suppress different functional groups of ruminal prokaryotes in the presence or absence of protozoa to evaluate changes in fermentation, digestibility, and microbial N outflow. Four dual-flow continuous culture fermenter systems were used in 4 periods in a 4 à 4 Latin square design split into 2 subperiods. In subperiod 1, a multistage filter system (50-µm smallest pore size) retained most protozoa. At the start of subperiod 2, conventional filters (300-µm pore ...