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Article: In vitro germination and micropropagation of Givotia rottleriformis Griff.
- Article from:
- In Vitro Cellular & Developmental Biology
- Article date:
- July 1, 2009
- Author:
CopyrightCopyright Society for In Vitro Biology Jul/Aug 2009. Provided by ProQuest LLC. (Hide copyright information)
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Abstract
The propagation of Givotia rottleriformis Griff. is difficult as a result of long seed dormancy associated with poor seed germination. The present study was undertaken to develop a protocol to overcome seed dormancy by culture of zygotic embryo axes and then develop an efficient method for micropropagation of Givotia. Best germination frequency (78.3%) was achieved from mature zygotic embryo axes isolated from acid-scarified fresh seeds when cultured on Murashige and Skoog (MS) medium (half-strength major salts) with 28.9 µM gibberellic acid (GA^sub 3^). Efficient plant conversion was achieved by transfer of 10-d-old germinated embryos to MS medium (half-strength major salts) ...