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Article: In vitro propagation of northern red oak (Quercus rubra L.)
- Article from:
- In Vitro Cellular & Developmental Biology
- Article date:
- July 1, 2009
- Author:
CopyrightCopyright Society for In Vitro Biology Jul/Aug 2009. Provided by ProQuest LLC. (Hide copyright information)
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Abstract
In vitro propagation of northern red oak (Quercus rubra) shoots was successful from cotyledonary node explants excised from 8-wk-old in vitro grown seedlings. Initially, four shoots per explant were obtained on Murashige and Skoog (MS) medium supplemented with 4.4 µM 6-benzylaminopurine (BA), 0.45 µM thidiazuron (TDZ), and 500 mg l^sup -1^ casein hydrolysate (CH) with a regeneration frequency of 64.7% after 3 wk. Subculturing explants (after harvesting shoots) to fresh treatment medium significantly increased shoot bud regeneration (16.6 buds per explant), but the buds failed to develop into shoots. A higher percentage (73.3%) of the explants regenerated four shoots per explant ...