Article: Long-term effects of histidine depletion on whole-body protein metabolism in healthy adults

Long-Term Effects of Histidine Depletion on Whole-Body Protein Metabolism in Healthy Adults1,2

ABSTRACT The essentiality of histidine in healthy adults is a controversial topic. To study the potential metabolic effects of a lack of exogenous histidine, four healthy adults consumed a histidine-free diet, with adequate energy and 1.0 g/(kg x d) of an L-amino acid mixture for 48 d. Protein metabolism was monitored every 4 d by using indicator amino acid (L-[1-^sup 13^C]phenylalanine) oxidation (in four subjects) and [^sup 15^N]glycine (in one subject). Urine samples (24-h) were collected for measurement of urea, total nitrogen, creatinine, 3-methylhistidine (3-MH), histidine and beta-alanine. ...

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