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Article: Auxin regulation of the gibberellin pathway in pea
- Article from:
- Plant Physiology
- Article date:
- December 1, 2002
- Author:
CopyrightCopyright American Society of Plant Physiologists Dec 2002. Provided by ProQuest LLC. (Hide copyright information)
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Auxin Regulation of the Gibberellin Pathway in Peal
The auxin indole-3-acetic acid (IAA) has been shown to promote the biosynthesis of the active gibberellin (GA,) in shoots of pea (Pisum sativum). We used northern analysis to investigate the timing of IAA-induced changes in transcript levels of PsGA3ox1 (Mendel's LE), PsGA2ox1, PsGA2ox2, and PsGA20ox1, key genes for the later stages of GA, biosynthesis and metabolism in pea. Rapid (2-4 h) changes occurred in the transcript levels of PsGA3ox1, PsGA2ox1, and PsGA2ox2 after treatent with IAA. [14C]GA, metabolism studies in decapitated shoots indicated that IAA inhibits GA, deactivation, consistent with the suppression of PsGA2ox1 (SLN) ...