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Article: Differential regulation of RNA levels of gibberellin dioxygenases by photoperiod in spinach
- Article from:
- Plant Physiology
- Article date:
- December 1, 2002
- Author:
CopyrightCopyright American Society of Plant Physiologists Dec 2002. Provided by ProQuest LLC. (Hide copyright information)
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Differential Regulation of RNA Levels of Gibberellin Dioxygenases by Photoperiod in Spinach1
Previous work with spinach (Spinacia oleracea) has shown that the level of gibberellin (GA) 20-oxidase is strongly upregulated by long days (LD). In the present work, the effect of photoperiod on expression of other GA dioxygenases was investigated and compared with that of GA 20-oxidase. Two GA 2-oxidases and one GA 3-oxidase were isolated from spinach by reverse transcription-polymerase chain reaction with degenerate primers and by 5'- and 3'-rapid amplification of cDNA ends. As determined by high-performance liquid chromatography with on-line radioactivity detection, the SoGA3ox1 gene product ...
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